Fig 2: miR-1290 targets and negatively regulates CDC73. (A) a volcanic map showing differentially expressed mRNAs in GSE86411; (B) a heatmap showing differentially expressed mRNAs in GSE86411; (C) the intersection of the target mRNA of miR-1290 predicted by TargetScan and miRDB websites and the mRNAs downregulated in the GSE86411 microarray; (D) RT-qPCR detection of CDC73 expression in cancerous and paired adjacent tissues from 57 patients with BC; (E) Pearson’s correlation analysis of CDC73 and circ_0000629 expression in 57 BC patients; (F) Pearson’s correlation analysis of CDC73 and miR-1290 expression in 57 BC patients; (G) expression of CDC73 in bladder uroepithelial cells and BC cell lines detected by RT-qPCR; (H) mRNA expression of CDC73 in T24 and SW780 after overexpression of circ_0000629 and/or miR-1290 mimic detected by RT-qPCR; (I) protein expression of CDC73 in T24 and SW780 after overexpression of circ_0000629 and/or miR-1290 mimic detected by Western blot; (J) the binding relationship between miR-1290 and CDC73 validated by dual-luciferase experiments. Date are mean ± SD, n = 3. ***p < 0.001 (paired t-test) or **p < 0.01 (two-way ANOVA and Tukey’s multiple comparison test).